Month: April 2018

Making Magic Happen: Surrey’s Best Wedding Photographers

Weddings are undoubtedly one of the most memorable and joyous occasions in a person’s life. They signify the union of two individuals in love, surrounded by wedding photographer surrey/ their closest family and friends. As such, capturing these precious moments through stunning photographs is of utmost importance. In Surrey, a picturesque county in the south-east of England, there are numerous talented wedding photographers who excel in making magic happen.

Surrey boasts a diverse range of landscapes, from rolling hills and lush green fields to historic castles and elegant manor houses. It is against this stunning backdrop that Surrey’s best wedding photographers work their magic, capturing the essence of love, joy, and happiness in every shot.

One such photographer is Sarah Adams, whose passion and creativity are evident in her work. With a keen eye for detail and a talent for capturing candid moments, Sarah’s photographs tell a story, weaving together the emotions and atmosphere of the day. Her ability to make couples feel at ease ensures that every couple she photographs feels comfortable and relaxed, resulting in natural and authentic images that will be cherished for a lifetime.

Another talented wedding photographer in Surrey is Michael Evans. With a background in fine art photography, Michael brings a unique and artistic approach to wedding photography. His ability to capture the beauty in the smallest details, combined with his expert use of light and shadows, creates dream-like images that evoke nostalgia and romance. Michael’s photographs not only capture the moments but also the emotions, creating a visual narrative that reflects the love between the couple.

For those seeking a more contemporary and fashion-forward style of wedding photography, Emma Harper is the go-to photographer in Surrey. With a background in fashion photography, Emma brings a fresh and modern perspective to wedding photography. Her ability to create striking compositions, combined with her skill in directing couples, results in editorial-worthy images that are both glamorous and timeless. Emma’s photographs are not just pictures, but works of art that speak to the couple’s personality and style.

In addition to individual photographers, there are also renowned wedding photography studios in Surrey that excel in making magic happen. One such studio is Capture the Moment, known for their exceptional storytelling and creativity. With a team of talented photographers, they collaborate to capture every aspect of the wedding day, from the intimate moments between the couple to the joyous celebrations with family and friends. Their ability to combine candid shots with beautifully orchestrated poses creates a stunning collection of images that tell the complete story of the day.

When it comes to choosing a wedding photographer in Surrey, it is crucial to find someone who not only possesses technical skill but also understands the importance of capturing emotions and creating a visual narrative. The photographers mentioned above are just a few examples of the many talented individuals in Surrey who excel in making magic happen.

No matter the style or approach, the best wedding photographers in Surrey have a common goal: to capture the essence of love and joy in every shot. Through their creativity, expertise, and passion, they immortalize one of the most special days in a couple’s life, allowing them to relive the magic for years to come.

Step-by-Step Guide to Gene Quantification Using qRT-PCR

Gene quantification is a crucial technique used in molecular biology to measure the expression levels of specific genes. Among the various methods available, quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a widely used and reliable approach. This article presents a step-by-step guide to successfully perform gene quantification using qRT-PCR.

Step 1: RNA extraction
The first step in qRT-PCR is to extract RNA from the samples of interest. This can be done using commercially available RNA extraction kits or by using a TRIzol reagent-based method. It is important to ensure that the RNA extracted is of high quality and free from any contaminants that may affect downstream analyses. Quantify the RNA concentration and assess its integrity using a spectrophotometer or a bioanalyzer.

Step 2: Reverse transcription
Reverse transcription is a process that converts RNA into complementary DNA (cDNA), which is more stable and can be amplified using PCR. Use a reverse transcription kit that includes a reverse transcriptase enzyme, primers targeting the gene of interest, and other necessary components such as nucleotides and buffers. Follow the kit instructions to reverse transcribe the RNA into cDNA.

Step 3: Primer design
Design gene-specific primers for the target gene and a reference gene. The target gene primers should anneal to a specific region of the gene, preferably in an exon-exon junction to avoid amplification of genomic DNA. The reference gene primers should target a housekeeping gene that is stably expressed across samples. Ensure that the primers have similar melting temperatures (Tm) to ensure efficient amplification.

Step 4: qPCR reaction setup
Prepare the qPCR reaction mix using a master mix or by combining a suitable combination of reagents. The reaction mix generally includes primers, cDNA template, a DNA polymerase enzyme, dNTPs, and reaction buffer. Use suitable positive and negative controls to validate the reaction. Prepare the reaction mix according to the manufacturer’s instructions.

Step 5: qPCR cycling conditions
Set up the qPCR instrument with appropriate cycling conditions. These conditions typically include an initial denaturation step at a high temperature, followed by a specific number of cycles consisting of denaturation, annealing, and extension steps. The optimal cycling conditions vary depending on the specific qPCR instrument being used and the primers designed.

Step 6: Data analysis
After running the qPCR reaction, the instrument will generate raw data in the form of amplification curves. Analyze the data using appropriate software, such as the comparative Ct (threshold cycle) method or the relative quantification method. The Ct value is the cycle where the fluorescence signal exceeds a predetermined threshold. Compare the target gene expression to the reference gene expression to determine the gene expression level.

Step 7: Data interpretation and validation
Interpret the data obtained from qRT-PCR analysis. Normalize the target gene expression to the reference gene expression to account for variations in overall RNA input and reverse transcription efficiency. Statistical analysis can be performed to assess significant differences in gene expression levels between samples or experimental conditions. Validate the results using additional assays, such as western blotting or gene quantification functional assays, if necessary.

In conclusion, qRT-PCR is a powerful technique for accurately quantifying gene expression levels. By following this step-by-step guide, researchers can perform gene quantification using qRT-PCR effectively and obtain reliable and reproducible results. It is important to ensure proper experimental design, appropriate primer selection, and accurate data analysis for successful qRT-PCR experiments.